We can distinguish between a (closed) Mobius strip and 'regular' (untwisted) strip by examining the set of points which have no neighborhood homeomorphic to a disk (intuitively, the 'boundary' of the strip). For a Mobius strip, this set is connected, while for an untwisted strip the set has two connected components.
However, the above criteria cannot distinguish between, say, a Mobius strip with 1 half-twist and a Mobius strip with 3 half-twists; it can only tell us if the number of twists is even or odd.
Can we tell how many half-twists a strip has in a purely topological way that avoids the embedding of the strip in $\mathbb{R}^3$? The more I think about it, the less convinced I am that we can. I can show all strips with an even number of half-twists are homeomorphic to $[0,1] \times S^1$ (by coordinatizing one of the circles as $\{0\} \times S^1$, the other as $\{1\}\times S^1$, and interpolating in between), but I'm not sure how or if I can adapt this approach to the case when there is an odd number of half-twists.