As Umberto P. already hints at, there are no uncountable subsets $E$ of $\Bbb{R}$ consisting only of isolated points; indeed, the intersection of $E$ with each $[-n,n]$ is finite (why?), so that $E$ is countable (why?).
EDIT: As @TonyK points out, the argument above is incorrect. But it is still true that every set consisting solely of isolated points is countable. To see this, note that each $x \in E$ has an (open) neighborhood $U_x$ in $\Bbb{R}$ such that $E \cap U_x$ is finite (in fact, one can choose $U_x$ so that $E \cap U_x = \{x\}$. Now $\Bbb{R}$ is second countable and hence every subset is separable, so that each subset is Lindelöf (see e.g. Prove that a separable metric space is Lindelöf without proving it is second-countable), so that there is a countable subcover $(U_{x_n})_n$. But then $E \subset \bigcup_n [E \cap U_{x_n}]$ is countable, because each $E \cap U_{x_n}$ is finite.
Also note that the definition of Lebesgue measure requires countable covers by intervals, not finite covers (otherwise, $\Bbb{Q}$ would not have measure zero).
An interesting set of positive (but smaller than expected) measure is obtained by
$$
\bigcup B(x_n, 1/2^n),
$$
where $(x_n)_n$ is an enumeration of $\Bbb{Q}$.