It’s true if you’re talking about well-ordered cardinals (assuming, of course, that $\lambda\ge\omega$). Let $\kappa$ and $\lambda$ be cardinals with $\kappa<\lambda$. Clearly $$f:\lambda\setminus\kappa\to\lambda:\xi\mapsto\xi$$ is an injection. The map
$$g:\lambda\to\lambda\setminus\kappa:\xi\mapsto\kappa+\xi\;,$$
where the addition is ordinal addition, is also an injection, so $|\lambda\setminus\kappa|=\lambda$ follows from the Schröder-Bernstein theorem.
In the absence of the axiom of choice you can have an amorphous set set $X$, an infinite set that is not the union of two disjoint infinite sets. Let $X_k=X\times\{k\}$ for $i\in\{0,1\}$, and let $Y=X_0\cup X_1$. Then $|X_0|<|Y|$, since there is no injection of $Y$ into $X$, but $$|Y\setminus X_0|=|X_1|=|X_0|\ne |Y|\;.$$
Added: In fact, as Asaf reminds me, the result is equivalent to the axiom of choice. Suppose that $X$ is a set that cannot be well-ordered, and let $\kappa$ be the Hartogs number of $X$: $\kappa$ is the smallest well-ordered cardinal that cannot be injected into $X$, and its existence is provable in $\mathsf{ZF}$. Let $Y=X\sqcup\kappa$. Then $|X|<|Y|$, but $Y\setminus X=\kappa$, and $|Y|>\kappa$, since there is no injection $Y\to\kappa$: if there were one, $X$ would be well-orderable.