Using the adjugate matrix and $\det(AB)=\det(A)\det(B)$, we can show that a matrix $A$ over a commutative ring $R$ is invertible iff $\det(A)\in R^{\times}$.
Yes. First we analyze a bit. If $Ax=\alpha x$, then $(A-\alpha I)\alpha=0$, hence $A-\alpha I$ is not invertible, equivalently $\det(A-\alpha I)$ is not invertible. When $R$ is not a field, this is not the same as to say $\det(A-\alpha I)=0$. In fact, we can find the almost trivial example $R=\mathbb Z/6\mathbb Z, n=1, A=2, x=3, \alpha=0$.
Yes. $\det(\alpha I - A)=0$ and $\det(\alpha I - B)=0$ imply $\det(\alpha\beta I - A\otimes B)=0$ is true for any field, and hence is true in the fraction field of $\mathbb Z[\{x_i\}_{i\in I}]$, hence hold in the ring itself and further in any of its quotient ring, which would be all rings.
To elaborate the last point a bit more. Consider the elements $p_1=\det(\alpha I - A), p_2=\det(\beta I - B), q=\det(\alpha\beta I - A\otimes B)$ as polynomials (over $\mathbb Z$) where all of $\alpha, \beta$ and entries of $A, B$ are just variables. Then we know that as polynomials over $\mathbb C$, $p_1=0$ and $p_2=0$ imply $q=0$ when all entries are replace by complex numbers. Therefore, by Hilbert's Nullstellensatz, $q^n=p_1'p_1+p_2'p_2$ over $\mathbb C$. And we may assume $p_1', p_2'\in \mathbb Z[\alpha, \beta, A_{ij}, B_{ij}]$. If we know $p_1, p_2$ generate a prime ideal, then we may assume $n=1$. That is $p_1=0$ and $p_2=0$ imply $q=0$ in the universal ring $\mathbb Z[\alpha, \beta, A_{ij}, B_{ij}]$, hence it must hold in any commutative ring $R$. Adapt this argument, we can show that $p_1, p_2$ are irreducible, and since they don't share any common variables, we know the ideal genrated by them is prime.