Consider the two structures $(\mathbb{R};f)$ and $(\mathbb{R};-)$, where $f$ represents additive inverse, and $-$ represents binary subtraction. Certainly, $f$ can be defined from $-$. Can $-$ be defined by $f$? I don't think it can, but I want a formal proof that no possible definition works.
Edit: It is very clear to me that $-$ can't be expressed by a term in $f$, but I am wondering if the graph of $-$ can be defined by a first-order formula in $f$.