If $f$ is absolutely integrable, that is $\int_0^\infty |f(x)| \, dx < \infty$, then the proof is easy.
In this case -- because $|e^{-\alpha x}f(x)| \leqslant |f(x)|$ for all $\alpha \geqslant 0$ -- the improper integral converges uniformly by the Weierstrass M-test. This implies that $I(\alpha) = \int_0^\infty e^{-\alpha x} f(x) \, dx$ is continuous and $\lim_{\alpha\to 0+}I(\alpha) = I(0)$.
More generally -- for example if the improper integral $\int_0^\infty f(x) \, dx$ is only conditionally convergent -- the result is true as a consequence of Abel's test for the uniform convergence of an improper integral. Note that $x \mapsto e^{-\alpha x}$ is monotone with respect to $x \in [0,\infty)$ and is uniformly bounded for all $x \geqslant 0$ and $\alpha \geqslant 0$ since $|e^{-\alpha x}| \leqslant 1$. Additionally, $\int_0^\infty f(x) \, dx$ is convergent by hypothesis and, therefore, uniformly convergent for $\alpha \geqslant 0$ (trivially since there is no dependence on $\alpha$). Both condition of Abel's test are met and the improper integral is uniformly convergent for $\alpha \geqslant 0$.
To see why $\lim_{\alpha\to 0+}I(\alpha) = I(0)$ is implied by uniform convergence note that for any $ \epsilon > 0$ there exists $C_1>0$ such that for all $c > C_1$ we have (by integrability of $f$)
$$\left|\int_c^\infty f(x) \, dx\right| < \frac{\epsilon}{3},$$
and there exists $C_2>0$ such that for all $c > C_2$ and all $\alpha \geqslant 0$ we have (by uniform convergence of $I(\alpha)$)
$$\left|\int_c^\infty e^{-\alpha x} f(x) \, dx\right| < \frac{\epsilon}{3}$$
Thus, for any $c > \max(C_1,C_2)$ we have
$$\left|\int_0^\infty e^{-\alpha x} f(x) \, dx - \int_0^\infty f(x) \, dx \right| \\\leqslant \left|\int_0^c e^{-\alpha x} f(x) \, dx - \int_0^c f(x) \, dx \right|+ \left|\int_c^\infty e^{-\alpha x} f(x) \, dx \right|+ \left|\int_c^\infty f(x) \, dx \right| \\ \leqslant \int_0^c |e^{-\alpha x} - 1||f(x)| \, dx + \frac{2\epsilon}{3} \\ \leqslant (1- e^{-\alpha c}) \int_0^c |f(x)| \, dx + \frac{2\epsilon}{3}$$
The first term on the RHS can be made smaller than $\epsilon /3$ by choosing $\alpha$ sufficiently close to $0$ and the result $\lim_{\alpha \to 0+} I(\alpha) = I(0)$ follows.