Another approach to the question: if $\lambda$ is a simple eigenvalue, meaning it has algebraic (and thus geometric) multiplicity $1$, then there's an invertible $P$ such that $A=P(\lambda\oplus A')P^{-1}$ for some $A'$. This is just a restatement of the existence of a right eigenvector $v=Pe_1$ such that $Av=\lambda v$.
Then, the left eigenvector corresponding to $\lambda$ is $u^T=e_1^T P^{-1}$, which gives you $u^T A=\lambda u^T$.
It follows that $u^T v=e_1^T P^{-1} Pe_1=1$, i.e. the vectors aren't orthogonal.
Being the eigenspaces one-dimensional, it's clear that any other choice of such eigenvectors just gives scalar multiples and doesn't change the result.
Example with diagonalisable non-degenerate $2\times 2$ matrix
Consider $A=\begin{pmatrix}-1&-2\\1&2\end{pmatrix}$, whose eigenvalues are $\lambda_1=0$ and $\lambda_2=1$, and we can write it as
$$A = \underbrace{\begin{pmatrix}-1&-2 \\ 1 &1\end{pmatrix}}_{\equiv P}
\begin{pmatrix}1&0\\0&0\end{pmatrix}
\underbrace{\begin{pmatrix}1&2 \\ -1 &-1\end{pmatrix}}_{= P^{-1}}
,$$
and thus $P e_1=\binom{1}{-1}$ is a right eigenvector of $\lambda=1$, and $e_1^T P^{-1}=(-1,-2)$ is a corresponding left eigenvector. These are different, but have unit (and thus nonzero) overlap.
Similarly, the eigenvectors corresponding to $\lambda=0$ are $P e_2=\binom{2}{-1}$ and $e_2^T P^{-1}=(1,1)$, and it's interesting to note how they're orthogonal to the right eigenvectors with different eigenvalues.
Any example with a non-degenerate diagonalisable matrix works like this: you write $A=PDP^{-1}$ for some diagonal $D$, and thus the right eigenvectors $Pe_j$ and the left eigenvectors $e_i^T P^{-1}$ are biorthogonal.
Counterexample with non-diagonalisable matrix
Consider as another eaxmple $A=\begin{pmatrix}1&1\\0&1\end{pmatrix}$. This has the only eigenvalue $\lambda=1$, with algebraic multiplicity $2$ and geometric multiplicity $1$, and is thus not diagonalisable.
While it remains true that both left and right eigenvectors correspond to the only eigenvalue $\lambda=1$, in this case we have $A e_1= e_1$ and $e_2^T A=e_2^T$. In other words, the left and right eigenvectors corresponding to the same eigenvalue are orthogonal. This shows that the assumption of having a simple eigenvalue is crucial to the statement.