You can rewrite things as
$${x^2-\tan^2x\over x^2\tan^2x}=\left(x-\tan x\over x^3 \right)\left(x+\tan x\over x \right)\left(x\over\tan x \right)^2$$
and apply L'Hopital to each piece separately. In particular,
$$\lim_{x\to0}\left(x\over\tan x \right)^2=\left(\lim_{x\to0}{x\over\tan x}\right)^2=\left(\lim_{x\to0}{1\over\sec^2x}\right)^2=1$$
after which we have
$$\lim_{x\to0}\left(x-\tan x\over x^3 \right)=\lim_{x\to0}\left(1-\sec^2x\over 3x^2 \right)=\lim_{x\to0}\left(-\tan^2x\over3x^2\right)=-{1\over3}\left(\lim_{x\to0}{\tan x\over x}\right)^2=-{1\over3}$$
(making use of a the trig identity $\tan^2x=\sec^2x-1$). Putting this together with
$$\lim_{x\to0}\left(x+\tan x\over x \right)=\lim_{x\to0}\left(1+\sec^2x\over 1 \right)=2$$
we get
$$\lim_{x\to0}\left(x^2-\tan^2x\over x^2\tan^2x\right)=-{1\over3}\cdot2\cdot1=-{2\over3}$$
The take-home lesson here is that some seemingly messy limit calculations can be carried out by breaking things into nice clean pieces. The trick is learning to recognize which pieces are nice and clean.
Remark: It's not really necessary (and some would even say it's inappropriate) to use L'Hopital to get ${\tan x\over x}\to1$ or ${x+\tan x\over x}\to2$ as $x\to0$. These limits are really just the definition of the derivative $f'(0)$ for $f(x)=\tan x$ and $f(x)=x+\tan x$, respectively.