Yes, it would. If $A$ and $B$ had the same cardinality, there would by definition by a bijection (and therefore an injection) $f:B\to A$. It follows immediately that if there is no such injection, then $|A|\ne|B|$.
Thus, if you have an injection from $A$ to $B$, then either there is an injection from $B$ to $A$, in which case $|A|=|B|$ by the Cantor-Schröder-Bernstein theorem, or there is no injection from $B$ to $A$, in which case $|A|<|B|$.
In your specific problem there are fairly obvious injections from $A$ to $B$ and to $C$. It is possible to find an injection from $B$ to $A$ directly, but that is not how I would approach the problem. I would (1) note that for each $n$ there is a bijection between the polynomials of degree $n$ and $\Bbb R^{n+1}$; (2) find a bijection between $\Bbb R$ and $\Bbb R^2$; (3) show by induction that $|\Bbb R^n|=|\Bbb R|$ for $n\in\Bbb Z^+$; and (4) use this to show that $|B|=|\Bbb N\times\Bbb R|=|\Bbb R|$. Depending on how much I had already proved about cardinalities, I could skip some of these steps.
I would not that there is an easy bijection between $C$ and $\wp(\Bbb R)$ and apply Cantor’s theorem to deal with $C$.