My question is, since we know that $T$ is symmetric, does that immediately give us that the formula for $T^*$ will be $T$ itself?
That $T$ is symmetric means not only that $D(T) \subseteq D(T^*)$ but also that $T^* x = Tx$ on $D(T)$, i.e. $T^*$ is an extension of $T$. In fact, this is a characterization:
Proposition. Let $T$ be densely defined with domain of definition $D$. Then the following are equivalent:
$\langle T x, y \rangle = \langle x, Ty \rangle$ for all $x,y \in D$.
$T \subseteq T^*$, i.e. $D(T) \subseteq D(T^*)$ and $T y = T^* y$ for all $y \in D$.
If one of the previous conditions is fulfilled one calls $T$ symmetric.
Proof. (1.) $\implies$ (2.): Let $y \in D(T)$. Then the linear functional $\varphi_T(x) := \langle Tx, y\rangle$ is continuous by Riesz-Frechet, since $\varphi_T(x) = \langle x, Ty \rangle$ by (1.). This gives $y \in D(T^*)$. But then, for all $x \in D$ we have
$$
\langle x, Ty \rangle = \langle Tx, y \rangle = \langle x, T^* y \rangle.
$$
Since $D$ is dense, this gives $Ty = T^*y$ and the claim (2.) holds.
The other implication is trivial. $\square$
This proof shows that $T^* = i \frac{d}{dx}$ on the domain of $D(T^*)$. Unless the operator $T$ is self-adjoint the domain of $T^*$ will be stricly greater than $D(T)$. Furthermore, there is already a definition of $D(T^*)$, namely
$$
D(T^*) := \{ y \in H \colon x \mapsto \langle T x, y \rangle \text{ is continuous }\}.
$$
I don't think there is a general rule for explicitely determining the domain of the adjoint. However in the case of the operator $T$ with domain $$D(T) := \{ f \in C^1[0,1] \colon f(0) = f(1) = 0\} \subseteq L^2[0,1]$$
we are lucky.
Claim. $D(T^*) = \mathrm{AC}[0,1]$ and $T^* = i \frac{d}{dx}$ on $D(T^*)$ (Note that the second statement is not automatic). Here $\mathrm{AC}$ denotes the absolutely continuous functions.
Proof. Let $f \in D(T^*)$ and $g := T^* f$. Consider the function
$$
F(t) := \int_0^t g(s) ds.
$$
Since $g \in L^2[0,1] \subseteq L^1[0,1]$, where the inclusion comes from the fact that we are dealing with a finite measure space, the function $F$ is absolutely continuous and hence a.e. differentiable with $ F' = g$ a.e. Hence, for all $h \in D(T)$ we have
$$
\langle Th, f \rangle
= \langle h, T^* f \rangle = \langle h, g\rangle
= \langle h, F' \rangle
= -\langle h', F \rangle \\
= -\frac{i}{i}\langle h', F \rangle
= i \langle Th, F\rangle = \langle Th , -iF\rangle.
$$
This gives us
$$
\langle Th, f + iF \rangle = 0,
$$
which leads to $f + iF \in (\operatorname{range} T)^\perp.$
By the fundamental theorem of calculus and the definition of $D(T)$, we know that
$$
\operatorname{range} T = \left\{ f \in \mathrm{C}[0,1] \colon \int_0^1 f(t) = 0 \right\} = \left\{ f \in \mathrm{C}[0,1] \colon \langle f, 1\rangle = 0 \right\}.
$$
This yields
$$
\overline{\operatorname{range} T} = \{1\}^\perp
$$
which in turn gives us
$$
\{\operatorname{range} T\}^\perp = \overline{\operatorname{range} T}^\perp = \{1\}^{\perp\perp} = \operatorname{span} \{1\}.
$$
Since $F$ and $1$ are absolutely continuous this gives us that $f = -iF + \alpha \cdot 1$ is absolutely continuous, i.e. $f \in \mathrm{AC}[0,1]$ and
$$
i \frac{df}{dx} = F' = g = T^* f.
$$
Now we show $D(T^*) \supseteq \mathrm{AC}[0,1]$.
Let $f \in \mathrm{AC}[0,1]$ and $f' \in L^2$ be the a.e. existing derivative. Then, like above we calculate for all $g \in D(T)$
$$
\langle Tg, f\rangle = i\langle g',f\rangle = -i\langle g, f'\rangle = \langle g, if' \rangle,i.e.
$$
the linear form $g \mapsto \langle Tg, f\rangle$ is continuous and hence $g \in D(T^*)$. $\square$