This is an exercise 2.3 from Harris's AG: A First Course:
Using the result of the exercise 2.2 ($A(\mathbb{A}^2 \setminus \{0\}) = K[x_1, x_2]$) show that $X = \mathbb{A}^n \setminus \{0\}$, $n > 1$, [as a quasi-affine variety] is not isomorphic [as in biregular] to any affine variety.
I have serious issues with this exercise.
I'm going to use the fact that regular mappings are Zariski continuous. I am also assuming that the underlying field $K$ is infinite.
Consider a regular isomorphism $f: X \to Y$ where $Y$ is an affine subvariety of $\mathbb{A}^m$ cut out by polynomials $h_i$, $i \in I$. By (a slight generalization of) ex. 2.2 we know that the components of $f$ are exactly restrictions of polynomials to $X$, we'll denote the extension of $f$ to the whole $\mathbb{A}^n$ as $\hat{f}$. But then $h_i \circ \hat{f} \vert_{\mathbb{A}^1}$ has infinite number of zeros, which means that the image of $0 \in \mathbb{A}^n$ lies in $Y$ as well.
Consider now a line $L \subset \mathbb{A}^n$ passing through both preimages of $\hat{f}(0)$. It is now sufficient to prove that $\hat{f} \vert_{X \cap L}$ is not an isomorphism onto its image.
I don't really know how to proceed. It is clear that $X \cap L$ is isomorphic to a hyperbola $xy = 1$, and thus we can reduce the problem to the one about affine varieties. However, I don't know how to prove that they are not isomorphic without using Euclidean topology (and thus assuming that $K = \mathbb{R}$ or $K = \mathbb{C}$. I guess I could look ahead and use the definition of a singularity, but it's so far ahead in the textbook it would be cheating. I can't think of a nice Zariski closed set that $\hat{f}$ would not pull back onto a Zariski closed set either, and I'm pretty sure one doesn't exist. So I'm left with having to study coordinate rings of the hyperbola and its image, and I don't know how to reason about the latter.
Am I on the wrong track with this exercise? I suspect that I am, yet I don't know any better way to go about it. Is there a hint I could use?