The usual way to do this is using Burnside's lemma, which says that the size of the quotient group is given by
$$
|X/G|=\frac{1}{|G|}\sum_{g\in G}|X^{g}|,
$$
where $|X^{g}|$ is the number of elements of $X$ fixed by $g$. Here $G$ is the cyclic group of order four, and each Tsuro tile can have no symmetry, or $180^{\circ}$ rotational symmetry, or full $90^{\circ}$ rotational symmetry. So the number of inequivalent Tsuro tiles is given by
$$
\frac{1}{4}\left(|X|+|X_{2}|+2|X_{4}|\right).
$$
When counting tiles with $180^{\circ}$ rotational symmetry, consider two adjacent sides. You can choose some of these spots to pair with their mirror partners; the remainder (which must be even) must be paired with each other, but each pairing can be done in $2$ different ways (you can pair a spot with a different spot on the same half or on the mirror half). Similarly, when counting tiles with $90^{\circ}$ rotational symmetry, you choose some spots to pair with their mirror partners, leaving an even number to pair with each other, and each such pairing can be done in $4$ different ways. Given $q$ spots, where $q$ is even, there are $(q-1)!!$ ways to pair them with each other. So if there are $n$ spots on each side, then
$$
|X| = (4n-1)!!,
$$
$$
|X_2|=\sum_{k\le n}2^{k}{{2n}\choose{2k}}(2k-1)!!,
$$
and
$$
|X_4|=\sum_{k\le n/2}4^{k}{{n}\choose{2k}}(2k-1)!!.
$$
For $n=2$, then
$$
|X|=7!!=105, \\ |X_2|=4\cdot 3!! + 2\cdot {{4}\choose{2}} + 1 = 25, \\ |X_4|=4+1=5,$$
so
$$
T_2 = \frac{1}{4}\left(105 + 25 + 2\cdot 5\right)=35.
$$
A straightforward Python implementation is:
def fac(n, k=1):
if n<=1: return 1
return n*fac(n-k, k)
def ch(m, n):
return fac(m)/(fac(n)*fac(m-n))
def T(n):
x = fac(4*n-1, 2)
x2 = sum([2**k * ch(2*n, 2*k) * fac(2*k-1, 2) for k in xrange(n+1)])
x4 = sum([4**k * ch(n, 2*k) * fac(2*k-1, 2) for k in xrange(n/2+1)])
return (x + x2 + 2*x4) / 4
The known results are reproduced:
>>> [T(n) for n in xrange(2,8)]
[35, 2688, 508277, 163715822, 79059439095, 53364540054860]