The cardinality of real numbers is indeed $2^{\aleph_0}$. However $\aleph_1$ is not defined as $2^{\aleph_0}$ (the cardinality of the continuum, often denoted $\mathfrak c$), but it is defined as the smallest cardinality strictly greater than $\aleph_0$. Whether $2^{\aleph_0}=\aleph_1$ (termed the continuum hypothesis) can neither been proven nor disproven from ZFC. You can of course add it as additional axiom to ZFC, and in that extended set theory, it is then indeed true. However you can also add the axiom $2^{\aleph_0}\ne\aleph_1$ (that is, the assumption that there exists a set whose cardinality lies strictly in between the natural and the real numbers) to ZFC and get another set theory in which the claim is false.
Now usually mathematicians assume ZFC, not ZFC+continuum hypothesis (nor ZFC+negation of the continuum hypothesis), therefore it is a false belief that this relation must be true.
It is however not a false belief that this relation is true; that is only an unprovable belief. There's nothing inconsistent with assuming it to be true; you cannot disprove it; however you also cannot prove it. But that's true of many believes.
However you cannot use it in a proof unless you explicitly specify it as precondition of what you want to prove ("assuming the continuum hypothesis is true, …").