Questions tagged [spectroscopy]

Use this tag for questions about spectroscopic methods in practice and in theory, especially where more specific tags for the individual method (such as [nmr-spectroscopy] or [ir-spectroscopy]) are not available.

Spectroscopy is the study of the interactions between matter and radiated energy. Experimentally, the results are returned as a function of wavelength versus amplitude (intensity), where the spectral lines represent the resonance frequency of the sample.

There are many different types of spectroscopy. Common spectroscopy techniques include:

to name a few.

Spectroscopic techniques are often used in chemistry to characterize a compound and determine its constituents. In biochemistry, spectroscopy is often used to study the conformers and physical structure of proteins and other biological molecules.

A subset of these topics is time-resolved spectroscopy, which uses ultra-fast laser pulses (on the pico or femtosecond timescale) to analyze transient species as they are forming. Time-averaged spectroscopy, such as an FT-IR encountered in an organic lab, provides an average IR spectrum of all the molecules in your sample, whereas ultra-fast spectroscopy can provide information on the structures of intermediates and even very short-lived transition states as a reaction proceeds along its reaction coordinate. Whilst transient UV/vis and IR spectroscopy is common, ultra-fast NMR is not possible due to the relative longevity of the beta state.

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Why is wavenumber used in IR spectroscopy rather than wavelength?

In IR spectroscopy, the $x$-axis is used to represent wavenumber, in $\mathrm{cm^{-1}}$. Why is wavenumber, equal to $1/\lambda$, used in place of wavelength, which is simply $\lambda$? Sources I’ve already found explain why it was chosen rather…
sqrtbottle
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What is the difference between infrared action spectroscopy and conventional IR spectroscopy?

I'm familiar with infrared spectroscopy generally, which probes mainly the vibrational behavior of molecules under study. However, I'm seeing references in various literature to 'infrared action spectroscopy' (e.g., here), which I'm having a hard…
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Lifetime measurement: double peak in instrument response function

I am measuring flourescence lifetimes with a picosecond pulse laser, using the LifeSpec II with an MCP detector. Instead of a single peak rapidly decaying, as would be expected, my IRF contains two peaks. I am suspecting some sort of reflection…
nordmarj
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Ring Strain and C=O Stretching Frequency

Why does $\ce{C=O}$ stretching frequency increase with ring strain? Why does conjugation decrease $\ce{C=O}$ ring strain? I am told that cyclopropanone exhibits a higher $\ce{C=O}$ stretching frequency than does cyclohexanone. I know that higher…
Dissenter
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Fourier transform for spectroscopy spectra?

I was thinking back to my chemistry major days and remembering the bunch of cool spectra we produced by various means (HNMR, IR, UV/Vis) and I remember all the cool 'self-interference' like couplets, triplets doublets, etc. My question is basically…
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Atomic emission spectroscopy

There is a sentence in my book I don't understand: Every atom or ion, which has one valence electron, will have double lines in the atomic emission spectrum. For example $\ce{Na}$ will produce lines at $589.0\:\mathrm{nm}$ and…
Ndrina Limani
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Nanoscopy Nobel Prize - why are all the tools in the visible light range?

It seems like all the new advances in molecular imaging/microscopy are in the visible light range of the EM spectrum. An example would be the most recent Nobel Prize for Chemistry. Is there some reason we are only looking for things in the visible…
jtoul
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Tabulated absorption spectra of greenhouse gases?

Are there any free sources of tabulated absorption spectra of greenhouse gases from UV/Vis to far infrared (say 70 micrometers)? This link shows the net absorption over the whole atmosphere (at least as far as I understand it), but I would be…
oliver
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Uv vis and fluorescence spectroscopy: sensitivity

Is there any reason why fluorescence spectroscopy is said to be more sensitive than Uv-Vis?
user43021
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Can dust or fingerprints interfere in ultra-violet spectroscopy

My question pertains generally to any form of spectroscopy, but I'll focus on UV spectroscopy. I am trying to get information on carrying out spectroscopy, but most of it is theoretical. Wikipedia is completely useless. Textbooks generally go into…
puk
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How much CO₂ should one expect in an IR spectrum?

I ran an IR spectrum ($\ce{KBr}$ pellet) and found a sharp absorbance at $2360 \text{cm}^{-1}$, which was unexpected. As far as I can tell, this indicates either a nitrile or $\ce{CO2}$. I know that there is no nitrile in my product. Would you…
Anthony
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difference between absorption spectroscopy and extinction spectroscopy

can anyone tell me the difference between absorption spectroscopy and extinction spectroscopy in terms of experiment? and how to get extinction spectroscopy, how to get absorption spectroscopy? Thank you so much.
Tianyu Zheng
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Wavelength of iPhone 4S camera light for visible spectroscopy calibration

I ordered a DIY spectroscopy kit from public-lab, however don't have access to a halogen lamp to calibrate the wavelengths. I'm wondering if it would somehow be possible to calibrate the wavelength using the iPhone camera light?
user4779
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Spectrometer vs Spectrometry vs Spectroscopy

How do you differentiate between the three? I read that "Spectrometry deals with the measurement of a specific spectrum. There are four primary types of spectrometers: Mass spectrometry 2. Neutron triple axis spectrometry 3. Ion-mobility…
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Why convert benzoate in soda to benzoic acid for spectroscopy experiment?

I did an experiment where I analyzed the amount of caffeine and benzoate in soda via spectroscopy. I've got the absorbances and did all the math for everything, but I'm having a hard time figuring out why we had to add an acid to protonate the…
Melanie Shebel
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