$$\forall i,\forall j\neq i,\quad\sum_{k=1}^n\frac{a_i-a_k}{a_i+a_k}\cdot\frac{a_j-a_k}{a_j+a_k}=0.$$
We can't have two different $a_i=0$ because of the denominators; but we can allow one $a_i=0$, if the terms $k=i$ and $k=j$ are excluded from the sum.
For $n=3$, these equations are easy to solve: \begin{align*} (1,2):\quad&\frac{a_1-a_3}{a_1+a_3}\cdot\frac{a_2-a_3}{a_2+a_3}=0\\ (1,3):\quad&\frac{a_1-a_2}{a_1+a_2}\cdot\frac{a_3-a_2}{a_3+a_2}=0\\ (2,3):\quad&\frac{a_2-a_1}{a_2+a_1}\cdot\frac{a_3-a_1}{a_3+a_1}=0. \end{align*} Indeed we just get $a_1=a_2=a_3$.
For $n=4$, the first of $6$ equations is
$$(1,2):\quad\frac{a_1-a_3}{a_1+a_3}\cdot\frac{a_2-a_3}{a_2+a_3}+\frac{a_1-a_4}{a_1+a_4}\cdot\frac{a_2-a_4}{a_2+a_4}=0.$$
(For the other $5$, just permute the indices.) I multiplied to clear the denominators, then added equations $(1,2)$ and $(3,4)$ to get
$$4(a_1a_2-a_3a_4)^2=0$$
and thus
$$a_1a_2=a_3a_4,\quad a_1a_3=a_2a_4,\quad a_1a_4=a_2a_3.$$
These imply that $a_1^2=a_2^2=a_3^2=a_4^2$; and we can't have $a_i=-a_j$, again because of the denominators. So $a_1=a_2=a_3=a_4$.
Does this continue for $n\geq5$?
If the variables are non-negative real numbers, then we can arrange them in order, $a_1\geq a_2\geq a_3\geq\cdots\geq a_n\geq0$; equation $(1,2)$ is then a sum of non-negative terms, so each term must vanish, which gives $a_2=a_3=\cdots=a_n$. Then equation $(2,3)$ has only its first term remaining, which gives $a_1=a_2$.
What if some of the variables are negative, or complex numbers?
We might define $b_{ij}=\dfrac{a_i-a_j}{a_i+a_j}$ to simplify the equations to $\sum_kb_{ik}b_{jk}=0$. Collecting these into an antisymmetric matrix $B$, we see that the system of equations is just saying that
$$BB^T=-B^2=B^TB=D$$
is some diagonal matrix. But I don't think this tells us enough about $B$ itself.
The defining equation for $b_{ij}$ can be rearranged to
$$a_j=\frac{1-b_{ij}}{1+b_{ij}}a_i$$
so in particular
$$a_3=\frac{1-b_{13}}{1+b_{13}}a_1=\frac{1-b_{23}}{1+b_{23}}a_2=\frac{1-b_{23}}{1+b_{23}}\cdot\frac{1-b_{12}}{1+b_{12}}a_1;$$
cancelling $a_1$,
$$(1+b_{31})(1+b_{23})(1+b_{12})=(1-b_{31})(1-b_{23})(1-b_{12});$$
expanding,
$$2b_{12}b_{23}b_{31}+2b_{12}+2b_{23}+2b_{31}=0.$$
In this process I divided by some things that might be $0$, but this resulting cubic equation is valid nonetheless.
I think we can dispense with $a_i$ now. In summary, we need to solve the system of equations \begin{align*} \forall i,\forall j,\quad&b_{ij}+b_{ji}=0\\ \forall i,\forall j,\forall k,\quad&b_{ij}b_{jk}b_{ki}+b_{ij}+b_{jk}+b_{ki}=0\\ \forall i,\forall j\neq i,\quad&\sum_kb_{ik}b_{jk}=0. \end{align*} Is the only solution $b_{ij}=0$?