I modify keyboard shortcut by defaults command.
For example,
# Disable "Search word in terminal man page index"
defaults write com.apple.symbolichotkeys AppleSymbolicHotKeys -dict-add 124 "<dict><key>enabled</key><false/><key>value</key><dict><key>parameters</key><array><integer>65535</integer><integer>26</integer><integer>262144</integer></array><key>type</key><string>standard</string></dict></dict>"
Change "Move focus to next window" shortcut to Alt + Tab
defaults write com.apple.symbolichotkeys AppleSymbolicHotKeys -dict-add 27 "<dict><key>enabled</key><true/><key>value</key><dict><key>parameters</key><array><integer>65535</integer><integer>48</integer><integer>524288</integer></array><key>type</key><string>standard</string></dict></dict>"
How can I enable to work them without restart OS?
I expect there are some Input Service and need to restart it.
ex. killall SomeInputService